Before filling one of these tubes with blood for a cholesterol test, you're supposed to keep your stomach empty. But that may not be necessary. Nancy Louie/iStockphoto.com hide caption itoggle caption Nancy Louie/iStockphoto.com Shots - Health News OK To Eat Before Rolling Up Sleeve For Cholesterol Test? November 13, 2012 For most people, a nonfasting cholesterol test will do just fine, a Canadian study suggests. A meal beforehand is unlikely to change key ratios of fats in the blood that doctors use to assess a person's risk for heart disease and stroke.